Secondary structure of the Neurospora crassa plasma membrane H+-ATPase as estimated by circular dichroism.

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The fluorescein isothiocyanate-binding site of the plasma-membrane H+-ATPase of Neurospora crassa.

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Chemical state of the cysteine residues in the Neurospora crassa plasma membrane H(+)-ATPase.

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Modification of the Neurospora crassa plasma membrane [H+]-ATPase with N,N'-dicyclohexylcarbodiimide.

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Location of a dicyclohexylcarbodiimide-reactive glutamate residue in the Neurospora crassa plasma membrane H+-ATPase.

The proton pump (H+-ATPase) found in the plasma membrane of the fungus Neurospora crassa is inactivated by dicyclohexylcarbodiimide (DCCD). Kinetic and labeling experiments have suggested that inactivation at 0 degrees C results from the covalent attachment of DCCD to a single site in the Mr = 100,000 catalytic subunit (Sussman, M. R., and Slayman, C. W. (1983) J. Biol. Chem. 258, 1839-1843). I...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1988

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)69044-1